The optimum pH invoice impact in the medium Trichoderma xylanase enzyme spinning on increased production

Introduction and purpose:Trichoderma is one of ascomycete mesophile fungi, which exist in all soils and are part of the majority of soil’s cultivable fungi. pH medium plays an important role in morphological changes in the organism, the organism growth and enzyme production plays. PH changes during growth of the organism on the stability of the enzyme produced effective. In organisms that grow in high pH or low extracellular enzymes that are secreted can also be active at pH up or down. In this study we aim to influence the optimum pH of the medium Trichoderma xylanase enzyme is spinning on increased production.Methods: We provided the standard Trichoderma reesei strain from the microbe collection of Iranian Research Organization for Science and Technology (IROST). Then, we measured the activity of its Xylanase enzyme using the standard method of DNSA. Next, for the sake of optimization, different parameters of the medium like carbon, nitrogen, and pH sources, at different pH and temperature of 35 ° C in 96 hours and the parent strains were cultivated.Results:The parental strain of Trichoderma reesei is featured by the xylanase enzyme activity of 2/59 U. the strain at the present of wheat bran, ammonium nitrate, yeast extract respectively as the carbon, inorganic nitrogen, organic nitrogen, and at 7/5 pH and with the average of 35 °C had the most amount of production,Our results can be seen at pH 5.7 and enzymes are most active at pH more acidic also see that the enzyme alkaline pH values are less than that this could be due to The strain used in alkaline pH of acidic grows greater. In 2008, Goyal and his colleagues conducted a study that concluded that Trichoderma Vyrydy at pH 4-5 / 3 is the highest xylanase production (Meenakshiسومین کنفرانس ملی و اولین کنفرانس بینالمللی پژوهشهای کاربردی درزیست شناسی3rd National & 1st International Conference in applied research on Biology www.Chem-Bio.ir 2Goyal et al., 2008). Also in 1993, Bailey and his colleagues in their study on Trichoderma xylanase producers were spinning at pH 7 (M. Bailey et al., 1993). The study, Billy species of Trichoderma was similar with our study also yielded the same results. But in the study of different species and different Goyal can therefore also be the reason and other reasons

کلیدواژه ها:

Trichoderma reesei ، Xylanase ، pH