Effects of Gonadotropin Releasing Hormone (GnRH) on bovine spermatogonial stem cell proliferation
سال انتشار: 1393
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 414
فایل این مقاله در 10 صفحه با فرمت PDF قابل دریافت می باشد
- صدور گواهی نمایه سازی
- من نویسنده این مقاله هستم
استخراج به نرم افزارهای پژوهشی:
شناسه ملی سند علمی:
JR_IJVST-6-2_001
تاریخ نمایه سازی: 11 تیر 1396
چکیده مقاله:
Identification of exogenous factors affecting spermatogonial stem cells (SSCs) proliferation in vitro, provides worthy ways to study the basic biology of the cells. The aim of this study was to investigate the effects of a GnRH analogue (alareline acetate) on SSCs colonization in short-term co-culture with sertoli cells. Five, three-month old Holstein male calves were used to isolate spermatogonial and sertoli cells. Testicular germ cell collection was made by enzymatic digestion methods. The cells were co-cultured in a 15 day period and in vitro effects of various doses (0.5, 1,2 and 4 μg/ml) of GnRHa on SSCs colonization were assessed. Effects of GnRHa on SSCs proliferation were dose dependent. In conclusion, it was demonstrated that 1 μg/ml GnRHa was the optimum dose for SSCs colonization in comparison with control group. The highest treatment dose (4 μg/ml GnRHa), negatively affected SSCs colonization in comparison with control group
کلیدواژه ها:
نویسندگان
Parviz Tajik
Department of Clinical Sciences, Faculty of Veterinary Medicine, Science and Research Branch, Islamic Azad University, Tehran, Iran
Hesam Kohsari
Department of Clinical Sciences, Faculty of Veterinary Medicine, Science and Research Branch, Islamic Azad University, Tehran, Iran
Babak Qasemi-Panahi
Department of Animal Sciences, Faculty of Agriculture, University of Tabriz, Tabriz, Iran.
Iraj Sohrabi-Haghdoost
Department of Pathology, Faculty of Veterinary Medicine, Science and Research Branch, Islamic Azad University, Tehran, Iran