شناسایی عوامل مایکوپلاسمایی تورم پستان گوسفندان و بزهای استان فارس

Milk samples from ewes and goats of Fars Province were examined between Mar.1997 to Sept.1998.The Samples were randomly collected from 610 flocks in 19 cities of the province. The Samples were used in microbiological tests in order to detect any Mycoplasma SPP.infection Therefore samples were transfered to Microbiology Laboratory and added to 2% PPLO broth tubes which were supplemanted with 20% sterilized horse serum and 1% Yeast Extract.To avoid contamination 1-10000 Thallium Acetate and 500 iU/mi Penicillin G were added to each tube. Then incubated in 37 c Contaminated samples were centrifuged for 10 min at 2500 r. p.m. and supernatant filtered by 0.45 micron pore sized milipore membrane filter. The postive samples subcultured in PPLO broth for decond time, then 0.5 ml of each positive PPLO broth cultured on enriched PPLO agar medium in order to get single colonies of Mycoplasma. After a week incubation at 37 c in a moist chamber suspected Mycoplasma colonies observed under stereomicroscope Smear of each culture stained by Giemsa staining methed in order to see pleomorphic micro organisms (mycoplasmas) Fermentation of different carbohydrates which is mentioned in tablo 1 was conducted in PPLO broth with 1 % of each sugar and phenol red as indicator. Urea and argeinine hydrolysis test were done the same as sugar fermentation test except PH of broth medium which was 7 instead of 7.5 Diferent biochemical broth mediums poured in tubes and inoculated with 1 mi of a 48h Mycoplasma cultures. The tubes were incubated at 37 C for 6 days and daily PH changes were checked. Control tubes of each test uninoculated remained. A difference in PH between inoculated and uninocalated tubes was considered as acid production (positive result) Table 1 also shows the results of biochemical tests. The results of biochemical tests show that 33.72 % of goats and 38.40% of sheep milk samples were infected by Mycoplasma SPP. The cause of Mycoplasma mastitis in sheep was M.agalactiae 100% but in goats were 95.6% M. agalaetiae and 4.4% M.myeoides subsp mycoides Also it is declaired that M.mycoides could be isolated only in Abadeh area in the northern of the Province.