Isolation, characterization, and effectiveness of bacteriophage Pɸ-Bw-Ab against XDR Acinetobacter baumannii isolated from nosocomial burn wound infection
محل انتشار: مجله علوم پایه پزشکی ایران، دوره: 24، شماره: 9
سال انتشار: 1400
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 226
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شناسه ملی سند علمی:
JR_IJBMS-24-9_012
تاریخ نمایه سازی: 24 شهریور 1400
چکیده مقاله:
Objective(s): With emergence of drug resistance, novel approaches such as phage therapy for treatment of bacterial infections have received significant attention. The purpose of this study was to isolate and identify effective bacteriophages on extremely drug-resistant (XDR) bacteria isolated from burn wounds. Materials and Methods: Pathogenic bacteria were isolated from hospitalized patient wounds in specialized burn hospitals in Iran, and their identification was performed based on biochemical testing and sequencing of the gene encoding ۱۶S rRNA. Bacteriophages were isolated from municipal sewage, Isfahan, Iran. The phage morphology was observed by TEM. After detection of the host range, adsorption rate, and one-step growth curve, the phage proteomics pattern and restriction enzyme digestion pattern were analyzed.Results: All isolates of bacteria were highly resistant to antibiotics. Among isolates, Acinetobacter baumannii strain IAU_FAL۱۰۱ (GenBank accession number: MW۸۴۵۶۸۰), which was an XDR bacterium, showed significant sensitivity to phage Pɸ-Bw-Ab. TEM determined the phage belongs to Siphoviridae. They had double-stranded DNA. This phage showed the highest antibacterial effect at ۱۵ °C and pH ۷. Analysis of the restriction enzyme digestion pattern showed Pɸ-Bw-Ab phage was sensitive to most of the used enzymes and based on SDS-PAGE, protein profiles were revealed ۴۳ to ۹۰ kDa.Conclusion: Considering the potential ability of the isolated phage, it had an antibacterial impact on other used bacterial spp and also strong antibacterial effects on XDR A. baumannii. Also, it had long latency and low burst size. This phage can be a suitable candidate for phage therapy.
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نویسندگان
Ladan Rahimzadeh Torabi
Department of Microbiology, Falavarjan Branch, Islamic Azad University, Falavarjan ۸۴۵۱۵/۱۵۵, Isfahan, Iran
Monir Doudi
Department of Microbiology, Falavarjan Branch, Islamic Azad University, Falavarjan ۸۴۵۱۵/۱۵۵, Isfahan, Iran
Nafiseh Sadat Naghavi
Department of Microbiology, Falavarjan Branch, Islamic Azad University, Falavarjan ۸۴۵۱۵/۱۵۵, Isfahan, Iran
Ramesh Monajemi
Department of Biology, Falavarjan Branch, Islamic Azad University, Falavarjan ۸۴۵۱۵/۱۵۵, Isfahan, Iran
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