The effect of omega- ۳ polyunsaturated fatty acids on endothelial tight junction occludin expression in rat aorta during lipopolysaccharide-induced inflammation

Objective(s): Occludin is essential for proper assembly of tight junctions (TJs) which regulate paracellular endothelial permeability. Omega-۳ polyunsaturated fatty acids (Ω-۳ PUFA) protect endothelial barrier function against injury. Materials and Methods: We examined anti-inflammatory effect of Ω-۳ PUFA intake (۳۰ mg/kg/day for ۱۰ days) on expression and location of occludin in the aorta of adult Wistar rats after a single dose of bacterial lipopolysaccharide (LPS, Escherichia coli, ۱ mg/kg). The ultrastructure of TJs after LPS administration was also investigated. We measured plasma levels of C-reactive protein (CRP), Malondialdehyde (MDA) and CD۶۸ expression and determined the total activity of NO synthase (NOS) in the aortic tissue. Results:LPS induced a significant decrease of occludin expression accompanied by structural alterations of TJs. Levels of CRP, MDA, CD۶۸ and NOS activity were elevated after LPS injection compared to controls indicating presence of moderate inflammation. Ω-۳ PUFA supplementation did not affect occludin expression in treated inflammatory group. However they reduced CRP and MDA concentration and CD۶۸ expression, but conversely, they increased NOS activity compared to inflammatory group. Conclusion:Our results indicate that a single dose of LPS could have a long-term impact on occludin expression and thus contribute to endothelial barrier dysfunction. ۱۰-day administration of Ω-۳ PUFA had partial anti-inflammatory effects on health of rats without any effect on occludin expression.

کلیدواژه ها:

aorta ، Endothelium ، Lipopolysaccharide ، Occludin ، Omega-۳ polyunsaturated fatty acids