Effect of hydro-alcoholic extract of Matricaria recutita L. on pentylenetetrazole-induced kindling and some biochemical parameters in male mice

Background: As Matricaria recutita (MAT) comprises a series of flavonoid compounds with benzodiazepine-like properties, it may be effective in the treatment of epilepsy and seizure disorders. In this study, the effect of intraperitoneal injection of hydro-alcoholic MAT extract on seizure induced by pentylenetetrazole (PTZ) in mice was evaluated.Method: Utilizing a PTZ-kindling model, ۴۸ male mice (۲۵-۳۰ g) were divided into ۶ groups (n= ۸): Saline, Control (PTZ-treated), MAT (۵۰ mg/kg), MAT (۴۰۰ mg/kg), Valproate (۱۵۰ mg/kg), Valproate (۵۰ mg/kg)+ MAT (۵۰ mg /kg). The seizure threshold was evaluated as a criterion for kindling. In addition, nitric oxide metabolites (NOx) and malondialdehyde (MDA) were measured in the brain tissue.Results: Since MAT enhanced the seizure threshold, it revealed an anticonvulsant property. Moreover, MAT significantly increased MDA levels in the brain, whereas it decreased the levels of NOx.Conclusion: The hydro-alcoholic extract of MAT has an inhibitory effect on PTZ-induced seizures. The extract also decreased and increased NOx and MDA levels in the brain, respectively.

کلیدواژه ها:

German chamomile ، Kindling ، Pentylenetetrazole ، Nitric oxide ، Malondialdehyde