Molecular Investigation of Staphylococcal Cassette Chromosome mec (SCCmec) Elements Isolated from Intensive Care Unit
سال انتشار: 1399
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 166
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شناسه ملی سند علمی:
JR_JIML-7-2_008
تاریخ نمایه سازی: 6 اسفند 1401
چکیده مقاله:
Background and Aims: Based on the results, Staphylococcus aureus is one of the serious infectious agents found in community and hospitals with remarkable potential for high morbidity and mortality around the globe. The present study was carried out for molecular investigation of methicillin-resistant Staphylococcus aureus strains and Staphylococcal Chromosomal Cassette mec (SCCmec) phenotypes isolated from the intensive care unit in Hazrat Fatemeh Zahra hospital of Isfahan.
Materials and Methods: A total of ۷۶ clinical wound samples were collected from Hazrat Fatemeh Zahra Hospital in Isfahan and evaluated by polymerase chain reaction (PCR) methods. The Methicillin resistance Staphylococcus aureus (MRSA) screening was performed by genotypic and phenotypic methods; also antibiotic resistance pattern was determined by using the disk diffusion method and related genes by PCR.
Results: Totally, ۵۳ (۶۹.۷%) out of ۷۶ clinical samples were positive for MRSA. Of the ۷۶ MRSA strains, ۳۹ (۶۳.۵۱%) were PVL positive (۵۱.۳%). The most commonly infected samples were collected from wounds (۴۰.۸%). The most commonly detected antibiotic resistance genes were mecA (۸۹.۶۱%), tetK (۸۸.۲۳%), tetM (۴۹.۱۵%) and msrA (۴۶.۹۳%). Resultantly, it was shown that MRSA has the highest level of resistance against methicillin (۹۸%), penicillin (۹۷.۲۴%), tetracycline (۸۹.۶۴%). It was also revealed that the most commonly detected SCCmec types in the MRSA strains are types II (۱۴.۵۳%) and III (۱۶.۸۲%).
Conclusions: In summary, this paper argues that the orderly surveillance of hospital-associated infections and initial management and supervision of the antibiotic resistance patterns are required to control the prevalence of MRSA.
کلیدواژه ها:
نویسندگان
فهیمه نوربخش
Medical Toxicology Research Centre, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
وجیهه نوربخش
Hazrat Fatemeh Zahra Hospitals, Depending on the Treatment and Management of Social Security in Isfahan, Isfahan, Iran.
سمانه برونی
Department of Biology, Nourdanesh Institutes of Higher Education, Meymeh, Isfahan, Iran.
الهه تاجبخش
Departments of Microbiology, Faculty of Basic Sciences, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran.
دانا دانشمند
Nosocomial Infection Research Center, Isfahan University of Medical Science, Isfahan, Iran.
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