Study of Release Behavior of Carboplatin from Modified Immunoglobulin Nanoparticles by Folic Acid: Preparation, Characterization and Analytical Approaches

The sustained release potential of bioactive materials and drugs is a major requirement in the development of carriers for cancer treatment. In this study, Carboplatin (Crb) as a standard anticancer drug was loaded to immunoglobulin G nanoparticles (IgGNPs) in the absence (Crb@IgGNPs) and the presence of folic acid (FA), ([e-mail]) as a targetable agent. Their physicochemical properties were characterized by various techniques. Dynamic light scattering (DLS) technique indicated that the average hydrodynamic diameter and zeta potential values of Crb@IgGNPs and [e-mail] were ۸۳۱.۲۳±۴.۹۵ nm; (PDI: ۰.۹۸۰.۳۱), ۳۹۷.۴۷±۲۲.۹۶ nm; (PDI: ۰.۷۸۰.۰۸) and -۲.۹۷±۱.۱۷ mV, -۷.۰۶±۰.۷۲ mV, respectively. The spherical shapes of the nanocarriers showed more particle size distribution in [e-mail] are confirmed by field emission scanning electron microscopy (FESEM) and atomic force microscopy (AFM). Fourier-transform infrared (FTIR) spectra of nanocarriers confirmed Crb loading onto IgGNPs and FA.IgGNPs. Afterwards, In vitro release study of Crb and [e-mail] was performed that demonstrated Crb was slowly released from FA.IgGNPs (about ۶۱ h longer than only Crb) and the release mechanism was followed by korsmeyer-peppas model with Fickian diffusion. Overall, it was observed that the novel designed drug carrier improved the drug release with the appropriate properties for clinical approaches.

کلیدواژه ها:

IgG ، Nanocarrier ، Carboplatin ، Folate ، release