The stability of Immunoglobulin Y (IgY) against streptococcal infection in tilapia aquaculture environment for the development of prophylactic and therapeutic agents

Immunoglobulin Y (IgY) can be used as a prophylactic and therapeutic agent for streptococcal infection in tilapia. This study explores the stability of IgY against water salinity and pH, temperature, and the presence of protease, which all stimulate the conditions of the digestive tract of tilapia. It was found that water salinity and pH levels of ۰, ۱۵, and ۳۰ ppt as well as ۶.۵, ۷.۵, and ۸.۵ respectively, with an incubation period of six days, and temperatures of ۲۵, ۴۵, and ۶۰°C for ۶۰ minutes did not affect the molecular weight of IgY and resulted in positive ELISA test results. Extreme conditions at ۸۰°C and incubation with the pepsin showed negative results in the ELISA test starting at minute ۴۰. However, from its incubation at ۸۰°C, IgY did not show any change in its molecular weight, as shown in the SDS PAGE gel. Trypsin also did not affect the molecular weight characteristics of IgY and showed a positive result in the ELISA test. However, IgY could not be detected after its treatment with pepsin.  Based on this study, it can be concluded that IgY could lose its biological function due to heating to ۸۰°C and exposure to pepsin. However, IgY is resistant to trypsin, water salinity, and pH levels in tilapia aquaculture.

کلیدواژه ها:

Aquaculture ، Biological stability ، Immunoglobulin Y (IgY) ، streptococcal infection ، Tilapia