Simultaneous use of thyme essential oil and disodium fumarate can improve in vitro ruminal microbial fermentation characteristics

Two trials were conducted to investigate the effects of disodium fumarate (DSF; ۰.۰۰, ۸.۰۰, ۱۰.۰۰ and ۱۲.۰۰ mM) and thyme essential oil (TEO; ۰.۰۰, ۱۰۰.۰۰, ۲۰۰.۰۰, ۳۰۰.۰۰ and ۴۰۰.۰۰ µL L-۱) solely and simultaneously (۱۰.۰۰ mM DSF along with ۱۰۰.۰۰, ۲۰۰.۰۰, ۳۰۰.۰۰ and ۴۰۰ µL L-۱ TEO) on in vitro ruminal fermentation of a ۵۰:۵۰ alfalfa hay to concentrate diet. The DSF and TEO did not affect crude protein disappearance, gas production, microbial crude protein synthesis and hydrogen recovery. The DSF addition linearly increased partitioning factor (PF) and molar proportion of propionate and decreased acetate: propionate ratio and methane production. Moreover, ۱۰۰.۰۰ µL L-۱ of TEO decreased ammonia nitrogen, total volatile fatty acids concentration and methane production and increased PF compared to the control. Results of the present study demonstrated that simultaneous use of DSF and TEO can cause a further decrease in methane production and linearly increase in the molar proportion of propionate and efficiency of feed use compared to DSF and TEO solely.

کلیدواژه ها:

Ammonia nitrogen ، Hydrogen recovery ، Volatile fatty acids