Heat Shock Proteins ۲۷, ۷۰ and ۹۰ Protein Expression in Gastric Adenocarcinoma: Prognostic, Predictive and Cellular Differentiation Significances

Background: Gastric cancer is the second global leading cause of death from cancer and the most common gastrointestinal cancer in Iran. This condition is usually diagnosed at advance stages where treatment options are limited. Recently, heat shock proteins (HSPs) have been reported to be overexpressed in a wide range of malignancies and considered as promising candidate biomarkers and therapeutic targets for gastric adenocarcinoma. The aim of the present study was to compare HSPs protein expression between non-tumoral and tumoral sections from patients with gastric adenocarcinoma and determine HSPs protein expression correlation with histological stage, tumoral grade and prognosis. Methods: Immunohistochemistry was used to assess the expression levels of HSP-۲۷, ۷۰ and -۹۰ proteins on both tumoral and non-tumoral (margin of tumor as control group) sections in ۸۰ patients with gastric adenocarcinoma. Further analyses were histology, grade and stage of tumor (Tumor, node, and metastasis), HSPs expression level, clinicopathological significances, and survival rate. Results: The expression of HSPs was significantly increased in tumoral sections compared with non-tumoral sections (P<۰.۰۰۱). The HSP۲۷ expression was correlated with tumors on the corpus of stomach (P=۰.۰۴۹). Patients younger than ۶۳ years revealed higher expression levels of HSP۷۰ (P=۰.۰۴۰). High expression levels of HSP۹۰ were further assessed in well-differentiated and intestinal types of tumors (P=۰.۰۰۹ and P=۰.۰۱۹). Overexpressed levels of HSP۲۷ and ۹۰ were associated with the reduced survival rate of patients (P=۰.۰۱۷ and P=۰.۰۱۸). Conclusion: HSP۲۷ and HSP ۹۰ are potential prognostic biomarkers of patients’ survival rate. Patients harboring positive HSP۲۷ and HSP ۹۰ expression display worse disease-free survival compared to those with negative HSP۲۷ and HSP ۹۰ expression. Differential expression of HSPs may play crucial roles in the initiation and progression of gastric cancer and can be exploited as future therapeutic targets.

کلیدواژه ها:

Gastric Cancer ، Heat shock protein ، Adenocarcinoma