An in-silico approach leads to exploringof correlation of hsa-miR-۱۸۶-۵p and IL-۲ gene in humancancers

Background: MicroRNAs (miRNAs) are classified as noncodingRNAs that play critical roles in a broad range of biologicalprocesses. Since the detection of dysregulated miR-۱۸۶,multiple studies have focused on the role of miR-۱۸۶ in pancreaticcancer and other cancers (such as Acute myeloid leukemia,Gastric cancer, Bladder cancer, Ovarian cancer, Cervical cancer,and Breast cancer). Studies have shown that miR-۱۸۶ influencescell proliferation, apoptosis, migration, invasion, cellcycle regulation, and intracellular metabolism in various cancers.Therefore, this miRNA can affect the pathogenesis of variouscancers through the modulation of different pathways suchas TGF-β۱/Smad, PI۳K/AKT signaling, and others. This studyaims to examine the structure and function of miR-۱۸۶ relatedto the IL-۲ gene in human cancers, to gain a better cognition ofits potential as a biomarker and a therapeutic target for cancers.Materials and Methods: We used the NCBI database to select a gene called "IL-۲ Gene". Then We used the TargetScan databaseand found that there are ۷۵ miRNA interactions with theIL-۲ gene. Next, we selected hsa-miR-۱۸۶-۵p and then studiedand predicted using online bioinformatic tools including miRBase,miRDB, OncomiR, and Cytoscape database.Results: Collectively, obtained data revealed the hsa-mir-۱۸۶-۵p bonded to the ۳'-UTR region of the IL-۲ gene (position۱۴۵-۱۵۱) through seven hydrogen bonds. also, has been shownthat hsa-miR-۱۸۶-۵p is located on chr۱p۳۱.۱. The desired microRNAlength includes ۲۲ nucleotides and its sequence was۵'-caaagaauucuccuuuugggcu-۳'.Conclusion: Taking into account the dysregulation of expressionof hsa-miR-۱۸۶-۵p in human cancers and its direct interactionwith the IL-۲ gene, it can be observed that this miRNA cansevers as a potent regulator of signaling pathways involved incancer progression. in addition, these results suggest that Variousbiological processes in human cancers are affected by miR-۱۸۶. therefore, this evidence highlights the potential value ofmiR-۱۸۶ in the diagnosis, prognosis, and treatment of humancancers.

کلیدواژه ها:

Cancer ، IL-۲ gene ، in-silico analysis ، miRNA