Bioinformatic investigation of miR-۵۴۳ inliver cancer

Introduction: MicroRNAs are important molecules in theregulation of gene expression, and by binding to the target microRNA,they decrease its expression. These molecules mayplay an oncogenic or tumor suppressive role in various cancers,including liver cancer. Liver cancer is the third most commonfatal cancer worldwide. The relationship of miR-۵۴۳ has beeninvestigated in various human cancers such as breast and cervicalcancer. However, the role of miR-۵۴۳ in hepatocellularcarcinoma has not been well studied. Therefore, in this study,we investigated the role of this microRNA in liver cancer usingbioinformatics analysis.Methods: To determine the role in liver cancer, the targetgenes of this microRNA need to be verified. For this purpose,the downstream target genes of miR-۵۴۳ were identified usingwell-known target gene prediction tools such as miRDB (https://mirdb.org/mirdb/index.html), MiRWalk (http://mirwalk.umm.uni/), and targetscan (https://www.targetscan.org/vert_۸۰/).These databases identify genes whose expression is altered bymiR-۵۴۳. By studying these genes, it is possible to identify thesignaling pathways activated by miR-۵۴۳ in liver cancer.Results: The results of our study showed that ۱۲۰۸ target geneswere predicted for miR-۵۴۳. Some miR-۵۴۳ target genes areinvolved in carcinogenesis, such as MAPK۱, TNFSF۱۱, F-boxprotein and TBC۱, which may provide a treatment option forliver cancer. The development and progression of cancer is oftenassociated with inflammation. Our results showed that miR-۵۴۳ target genes such as IL-۱, LIF receptor and IRF۵ were alsoinvolved in the inflammatory process. Therefore, the role ofthese microRNA in liver cancer is more important.Conclusion: Considering that the expression of miR-۵۴۳ is alteredin liver cancer, the identification of its target genes maydetermine the pathogenic mechanism of this miR-۵۴۳.

کلیدواژه ها:

MiR-۵۴۳ ، Liver cancer ، bioinformatics ، Gene regulation