Preventive effects of quercetin against inflammation and apoptosis in cyclophosphamide-induced testicular damage
محل انتشار: مجله علوم پایه پزشکی ایران، دوره: 27، شماره: 5
سال انتشار: 1403
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 40
فایل این مقاله در 10 صفحه با فرمت PDF قابل دریافت می باشد
- صدور گواهی نمایه سازی
- من نویسنده این مقاله هستم
استخراج به نرم افزارهای پژوهشی:
شناسه ملی سند علمی:
JR_IJBMS-27-5_015
تاریخ نمایه سازی: 19 اسفند 1402
چکیده مقاله:
Objective(s): We aimed to investigate the effects of quercetin (QRC) against cyclophosphamide (CP)-induced testicular damage and how it interacts with apoptotic and inflammatory signaling pathways.Materials and Methods: Forty male Wistar rats were randomly divided into four groups, ۱۰ in each group; Control group (corn oil, intragastrically, ۱۴ days), QRC group (۱۰۰ mg/kg QRC, dissolved in corn oil, ۱۴ days), CP group (۲۰۰ mg/kg CP, intraperitoneally, single dose on the ۷th day), and CP+QRC group (۱۰۰ mg/kg QRC, intragastrically, ۱۴ days and ۲۰۰ mg/kg CP, intraperitoneally, single dose on the ۷th day). Animals were sacrificed one day after the last QRC application and the effects of quercetin were evaluated by histological, morphometrical, and hormonal parameters. Also, nuclear factor kappa B (NFkB), nuclear factor erythroid ۲ related factor ۲ (Nrf۲), Bcl-۲ associated X protein (Bax), and B-cell lymphoma-۲ (Bcl-۲) immunoreactivities were evaluated immunohistochemically.Results: CP increased the testicular weight/body weight ratio, significantly decreasing body weights and testicular weights. All hormone levels were also reduced significantly. Morphometrically, seminiferous tubules diameter and germinal epithelial thickness decreased, while a significant increase was determined in interstitial field width in addition to histological damage. Furthermore, immunohistochemical findings also indicated that NFkB and Bax immunoreactivity were increased in the CP group, whereas significant decrease was seen in Nrf۲ and Bcl-۲ immunoreactivity. Apoptotic cell and tubule index were reduced in CP. QRC ensured improvement in all findings.Conclusion: Data showed us, that QRC may have preventive effects in CP-induced testicular damage by acting on NFkB, Nrf۲, Bax, and Bcl-۲ pathways.
کلیدواژه ها:
نویسندگان
Duygu Uzun-Goren
Department of Histology and Embryology, Faculty of Medicine, Trakya University, Edirne, Turkey
Yesim Hulya Uz
Department of Histology and Embryology, Faculty of Medicine, Trakya University, Edirne, Turkey
مراجع و منابع این مقاله:
لیست زیر مراجع و منابع استفاده شده در این مقاله را نمایش می دهد. این مراجع به صورت کاملا ماشینی و بر اساس هوش مصنوعی استخراج شده اند و لذا ممکن است دارای اشکالاتی باشند که به مرور زمان دقت استخراج این محتوا افزایش می یابد. مراجعی که مقالات مربوط به آنها در سیویلیکا نمایه شده و پیدا شده اند، به خود مقاله لینک شده اند :