بررسی جداسازی، شناسایی و حساسیت آنتی بیوتیکی جدایه های اورنیتوباکتریوم رینوتراکیال از ماکیان تجاری شهرستان رشت

‭The aim of the present study was conducted Seroprevalence, Isolation, Determination of antibiotic sensitivity and Molecular diagnosis of Ornithobacterium rhinotracheale by PCR method. In this study, 460 serum samples were collected from 29 slaughtered broiler flocks. Serological results showed that 5 flocks (17/2%) were positive to ORT infection by Elisa, but in each individual flock of the birds only Ornithobacterium rhinotracheale antibodies in 24 serums out of 460 serum samples were positive (5/12%). ‬‮‭30‬ ‭Total of 290 tracheal swabs (29 samples) were collected. The causative agent ORT was isolated from tracheal swabs of 3 flocks (10/3% or 1/ 03 out of 290 tracheal swabs) by culture and with using specific primers of ORT in PCR method was amplified A 784 bp fragment of the 16SrRNA gene. By statistical, there was no significantly different between correlation of the isolates and flocks ORT Titers (P> 0/05). Drug sensitivity test using standard disk diffusion technique was performed with 13 antibiotics. All the isolates were resistant (100%) to Erythromycin, Tetracycline,Oxytetracyclin, Enrofloxacin, Ciprofloxacin, Flumequin, Lincospectin, Furazolidon and 100% of the Isolates were susceptible to Ceftriaxon and Tiamulin but 2 isolates (66/7%) were moderate sensitive to Tylosin and Amoxicillin and Sensitive to Flurfenicle. Key words: Isolation, Identification, Antibiotic , Ornithobacterium Rhinotracheale, Poultry‬

کلیدواژه ها:

اورنیتوباکتریوم رینوتراکیال ، جداسازی ، خواص آنتی بیوتیکی ، طیور