بررسی مقایسه روش ‭Dialysis culture ‬و ‭Batch culture ‬و مطالعه عوامل ایمنی زایی آن

Pertussis, a severe and highly contagious respiratory disease, is caused by Bordetella pertussis. Prior to the availability of vaccin~, pertussis was a common cause of morbidity and mortality among children. The whole cell pertussis was available in the early 20th century. Whole-cell vaccine has been very effective against the disease (Cherry 1984, Fine et a!. 1987). However, it was temporally associated with many side effects (Gold 1985, Edward et a1. 1999). These adverse side effects and public concerns have resulted to introduce acellular pertussis vaccines. Considerable evidences indicated that pertussis toxin (PT) is a major virulence factor ofB. pertussis and is important for immunity to disease (Pittman 1979,.Smith et al. 2001). In this study, chemically defined medium (CDM) was applied to find the best condition for pertussis toxin (PT) production. Both B. pertussis strains 134 and 509 were examined to determine their ability to PT production. Data collectively resulted to use of B. pertussis 509 in chemically defined medium as preferred strain for fermentation and PT production studies. VdialysateNfermenter ratio. In despite of increase of pertussis toxin, the levels of PT/OD were decreasing in dialysis fermentations. It may be attributed to antigenic modulations, which decrease virulent factors including PT, in response to environmental changes. As our study is the first reported attempt of dialysis fermentation ofB. pertussis, further efforts are necessary to explain the low efficacy of this approach. The results collectively indicate that, although dialysis fermentation is applicable for PT production aiming to provide an acellular pertussis vaccine, this approach is not appropriate for making a whole.cell pertussis vaccine.

کلیدواژه ها:

سیاه سرفه ، روش ‭Dialsys culture ، ‬روش ‭Batch culture ، ‬باکتری ‭Bordetella pertussis ، ‬واکسن(ها) ، کشت دیالیزی