Antimalarial and cytotoxic activities of roots and fruits fractions of Astrodaucus persicus extract

Objective(s):Astrodaucus persicus (Apiaceae) is one of the two species of this genus which grows in different parts of Iran. Roots of this plant were rich in benzodioxoles and used as food additive or salad in Iran and near countries. The aim of present study was evaluation of antimalarial and cytotoxic effects of different fractions of A. persicus fruits and roots extracts. Materials and Methods: Ripe fruits and roots of A. persicuswere extracted and fractionated by hexane, chloroform, ethyl acetate and methanol, separately. Antimalarial activities of fractions were performed based on Plasmodium berghei suppressive test in mice model and percentage of parasitemia and suppression were determined for each sample. Cytotoxicity of fruits and roots fractions were investigated against human breast adenocarcinoma (MCF-۷), colorectal carcinoma (SW۴۸۰) and normal (L۹۲۹) cell lines by MTT assay and IC۵۰ of them were measured. Results: Hexane fraction of roots extract (RHE) and ethyl acetate fraction of fruits extract (FEA) of A. persicus demonstrated highest parasite inhibition (۷۳.۳ and ۷۲.۳%, respectively at ۵۰۰ mg/kg/day) which were significantly different from negative control group (P<۰.۰۵). In addition, RHE showed potent anticancer activities against MCF-۷ (IC۵۰ of ۰.۰۱ µg/ml), SW۴۸۰ (IC۵۰ of ۰.۳۶ µg/ml) and L۹۲۹ (IC۵۰ of ۰.۷۰ µg/ml) cell lines. Conclusion: According to the results, RHE and FEA fractions of A. persicus could be introduced as excellent choice for antimalarial drug discovery. In addition, cytotoxic activity of RHE was noticeable.

کلیدواژه ها:

Antimalaria ، Apiaceae ، Astrodaucus persicus ، Cytotoxic ، MTT Assay ، Plasmodium berghei