Alkyl cross-linked low molecular weight polypropyleneimine dendrimers as efficient gene delivery vectors
محل انتشار: مجله علوم پایه پزشکی ایران، دوره: 19، شماره: 10
سال انتشار: 1395
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 281
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شناسه ملی سند علمی:
JR_IJBMS-19-10_009
تاریخ نمایه سازی: 30 مهر 1400
چکیده مقاله:
Objective(s): First, ۱۰-bromodecanoic acid was covalently attached to all available surface primary amines of PPI G۲ and G۳ to increase their lipophilicity. In the subsequent step, PPIs were conjugated to the alkylcarboxylate groups of alkylcarboxylate-PPI derivatives to increase the number of surface primary amines. Physicochemical properties of modified PPIs were determined. Transfection experiments (using both luciferase and green fluorescent protein (GFP)- expressing plasmids) and cytotoxicity assay were performed to evaluate the efficiency of the final derivatives. Materials and Methods: First, ۱۰-bromodecanoic acid was covalently attached to all available surface primary amines of PPI G۲ and G۳ to increase their lipophilicity. In the subsequent step, PPIs were conjugated to the alkylcarboxylate groups of alkylcarboxylate-PPI derivatives to increase the number of surface primary amines. Physicochemical properties of modified PPIs were determined. Transfection experiments (using both luciferase and green fluorescent protein (GFP)- expressing plasmids) and cytotoxicity assay were performed to evaluate the efficiency of the final derivatives. Results: Fabricated vectors condensed DNA effectively so that polyplexes with appropriate size (below ۱۵۵ nm) and positive surface charge were constructed. Cross-linked low molecular weight PPIs (G۲ or G۳) with decanoate linkage increased transfection efficiency significantly while maintaining the low cytotoxicity. PPI G۲ derivative exhibited increased buffering capacity which is believed to be responsible for better proton sponge mechanism leading to higher transfection efficiency. Conclusion: Our results indicated that oligomerization of low molecular weight PPI (PPI G۲-alkyl-PPI G۲ conjugate) could be an approach to increase the transfection efficiency and to lower the cytotoxicity of low molecular weight polycations.
کلیدواژه ها:
نویسندگان
Faezeh Moghadam Ariaee
Pharmaceutical Research Center, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran
Maryam Hashemi
Nanotechnology Research Center, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran
Sara Amel Farzad
Pharmaceutical Research Center, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran
Khalil Abnous
Pharmaceutical Research Center, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran
Mohammad Ramezani
Nanotechnology Research Center, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran
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