Surveying miRNAs and long non-coding RNAs in the glycolysis and impedance stages in cisplatin-treated MCF۷ cells

Introduction: Breast cancer is one of the most common and highly malignant cancers and the second leading cause of cancer death in women worldwide. Treatment with cisplatin, because of its pro-apoptotic characteristics, is one of the front line of anticancer strategy. Despite in-depth molecular knowledge regarding the impacts of cisplatin on the MCF۷ cell lines, there is no reports on its effects on the dynamic changes of non-coding RNAs during glycolysis and impedance steps. Methods: We have analyzed freely available microarray data to find differentially expressed miRNA and lncRNA. Our search strategy applied p-value (< ۰.۰۵) and a log۲ Fold change (> ۰.۷) as the threshold for data analysis. Next, we have categorized and further investigated the family and targets of these non-coding RNAs. Results: Of the ۲۳ common non-coding RNAs (۵ miRNA and ۱۸ lncRNA), in both glycolysis and impedance stages, three genes were upregulated. These include one miRNA and one lncRNA in the impedance stage and only one lncRNA gene in the glycolysis stage. Interestingly, two genes were upregulated at the impedance stage but further downregulated at the glycolysis stage. Conclusion: We were able to catalogue the non-coding RNAs and their expression patterns at two very important stages of cisplatin treated- MCF۷ cells.

کلیدواژه ها:

Cisplatin ، miRNAs ، lncRNAs