Vanadyl Sulfate has a Cytotoxic and Apoptotic Effect on MCF-۷ Breast Cancer Cell Line

Introduction & Aim: Breast cancer is the major cause of death from cancer among women. Some studies have indicated the cytotoxic effects of vanadyl oxide sulfate (VOSO۴). This study was done to evaluate the anti-cancer effect of VOSO۴ in the treatment of breast cancer. Methods: MCF-۷ cell line was treated with different concentrations of VOSO۴ for ۲۴ and ۴۸ hours. The measurement of cell death was performed by MTT assay. The cell apoptosis rate was measured using Annexin V / Propidium Iodide Assay through flow cytometry. The expression levels of p۵۳, P۲۱, Caspase۸, Sod۱, Sod۲, and Bcl۲ mRNAs were evaluated, and Western blotting was performed for Sod۱ to confirm the results. Results: The results showed that the half-maximal inhibitory concentration (IC۵۰) for VOSO۴ was ۲۵ and ۲۰ for ۲۴ and ۴۸ h, respectively. Indeed, VOSO۴ has dose-dependent cytotoxic effects on the MCF-۷. Also, the apoptosis of the cells after ۲۴ h of exposure to VOSO۴ was more than ۵۲%. Moreover, after ۲۴ hours of exposure to VOSO۴ with IC۵۰ concentration, the expression of p۵۳, P۲۱, Caspase۸, Sod۱, and Sod۲ mRNAs increased and the expression of Bcl۲ mRNA decreased. The Western blotting on Sod۱ confirmed these findings. Conclusion: Our results demonstrated that VOSO۴ has an apoptotic and cytotoxic effect on breast cancer cells. Therefore, it is likely that this substance can be considered as a complementary agent for the medical treatment of breast cancer patients.

کلیدواژه ها:

Vanadyl Sulfate ، Breast Cancer ، Anti-cancer ، MCF-۷ ، Anti-proliferative ، Apoptosis ، Cell Line