Interleukin-۱ beta, interferon-gamma, and tumor necrosis factor-alpha gene expression in peripheral blood mononuclear cells of patients with coronary artery disease

BACKGROUND: Several inflammatory mediators have been proposed to contribute to the pathogenesis of atherosclerosis. The aim of this study was to evaluate the quantitative expression of pro-inflammatory cytokines in un-stimulated peripheral blood mononuclear cell of patients with coronary artery disease (CAD). METHODS: Interleukin-۱ beta (IL-۱β), tumor necrosis factor-alpha, and interferon-gamma (IFN-γ) gene expression were evaluated in angiography confirmed patients with and without CAD in a case-control study using quantitative real-time polymerase chain reaction. RESULTS: A significant increase (P = ۰.۰۳۰) in IL-۱β gene expression was found in patients with CAD [median interquartile range (IQR) = ۴.۸۹۰ (۶.۰۸۴)] compared to patients without CAD [median (IQR) = ۱.۷۹۲ (۳.۱۷۲)]. Despite the increase in IFN-γ gene expression in patients with CAD [median (IQR) = ۱.۲۹۸ (۳.۸۹۶)] versus patients without CAD [median (IQR) = ۰.۸۴۱ (۲.۷۹)], there was not statistically significant difference (P = ۰.۹۹۰). CONCLUSION: Our results provide evidence for possible association between IL-۱β and development of atherosclerosis as a crucial cytokine that induce a network of signaling pathways. This finding if proved in future would suggest IL-۱β as a potent therapeutic target in CAD. 

کلیدواژه ها:

Coronary Artery Disease ، Interleukin-۱ Beta ، Tumor Necrosis Factor-alpha ، Interferon-gamma ، Gene Expression