Analysis of Genetic Diversity in Improved Varieties and Iranian Landraces of Alfalfa Using EST, POX, GOT and MDH Allozyme Markers
محل انتشار: مجله فیزیولوژی و پرورش گیاهان، دوره: 2، شماره: 2
سال انتشار: 1391
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 123
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شناسه ملی سند علمی:
JR_BREDNG-2-2_005
تاریخ نمایه سازی: 14 اسفند 1401
چکیده مقاله:
Genetic diversity of five improved varieties (Kaysari, Kadi, Ranger, Mesmir, Sea-River) and seven Iranian landraces (Gharayonje, Amozeynadin, Rahnani, Tazekand, Shazand, Hamedani, Yazdi) of alfalfa were assessed using Esterase (EST), Peroxidase (POX), Glutamate Oxaloacetate Transaminase (GOT) and Malate Dehydrogerase (MDH). GOT and MDH were monomorphic, whereas EST and POX showed polymorphic banding patterns. In total, four allozyme loci were detected for EST and POX. There were no significant differences between improved varieties and landraces for allelic frequency in the polymorphic markers. However, average heterozygosity in landraces (۰.۶۲۳) was higher than that of improved varieties (۰.۵۹۴). Genetic distance among populations was less than ۰.۰۲. FST of landraces versus improved varieties was ۰.۰۰۳ showing no differentiation based on the studied marker systems. The value of FIS was about ۰.۰۸۹, which indicated that inbreeding effect was larger than the genetic drift in alfalfa. Applying test showed that Hamedani was significantly different from nine varieties and Shazand from seven varieties. Based on cluster analysis, alfalfa landraces and improved varieties were not arranged in separate groups. This means that there were not appreciable differences between these groups for allozyme markers
کلیدواژه ها:
نویسندگان
Maryam Ahmadi
Former MSc student of Plant Breeding, Dept. of Plant Breeding and Biotechnology, University of Tabriz, Tabriz, Iran
Mostafa Valizadeh
Professor, Dept. of Plant Breeding and Biotechnology, University of Tabriz, Tabriz, Iran
Mahmood Toorchi
Professor, Dept. of Plant Breeding and Biotechnology, University of Tabriz, Tabriz, Iran
Mohammad Moghaddam
Professor, Dept. of Plant Breeding and Biotechnology, University of Tabriz, Tabriz, Iran
Hossein Mohammadzadeh Jalaly
Former MSc student of Plant Breeding, Dept. of Plant Breeding and Biotechnology, University of Tabriz, Tabriz, Iran