Comparison of the Modulated Effects of Tretinoin and Calcitriol Treated Mesenchymal Stem Cell Supernatant on Macrophage Functions

Background: According to several studies, Calcitriol and Tretinoin can regulate differentiation and the growth of mesenchymal stem cells (MSCs). Nevertheless, the relationship between the supernatant of macrophage and mesenchymal stem cells is still under investigation. In the present work, a comparison is made between the modulated impacts of Calcitriol and Tretinoin treated mesenchymal stem cell supernatant on macrophage functions. Materials and Methods: The isolation of mesenchymal stem cells was done using mouse bone marrow, and the various concentrations of Calcitriol (۲۰۰ and ۴۰۰ nM) and Tretinoin (۲۵, ۵۰, and ۱۰۰ nM) were used to pulse MSCs for ۴۸ h. Macrophages were then applied to co-culture the supernatant of MSCs for ۴ hr. Consequently, macrophages were assessed for respiratory burst. Results: Based on the obtained results, supernatant of bone marrow‐derived MSCs pulsed with Calcitriol and Tretinoin can have the potential for decreasing the respiratory burst of macrophages considerably in comparison with the control group. Conclusion: The anti‐inflammatory M۲ macrophage polarization can be accelerated using Calcitriol and Tretinoin by mesenchymal stem cells.