Synthesis of New Benzothiazole Derivatives and Evaluation of Cytotoxicity of N-(۶-Substitued-۱,۳-benzothiazol-۲-yl)-۴-phenyl-۱,۳-thiazol-۲(۳H)-imine Compounds

Cyclization of unsymmetrical thioureas affords N-(۶-substitued-۱,۳-benzothiazol-۲-yl)-۴-phenyl-۱,۳-thiazol-۲(۳H)-imine compounds by the reaction of ۱-(۱,۳-benzothiazol-۲-yl)thiourea derivatives and ۲-bromoacetophenone in the presence of Et۳N. Also, methyl-۱,۳-benzothiazol-۲-yl carbamodithioate derivatives with methyl anthranilate were reacted and ۳-(benzo[d]thiazol-۲-yl)-۲-thioxo-۲,۳-dihydroquinazolin-۴(۱H)-one derivatives as new compounds are synthesized. In the following, synthesized compounds (۲a- ۲d) were evaluated for anti-tumor activity against MCF-۷, MAD-MD-۲۳۱, HT-۲۹, HeLa, Neuro-۲a, K-۵۶۲ and L-۹۲۹ cell lines and the results obtained from MTT-assay revealed the best cytotoxicity for ۲b compound containing a phenolic segment in their buildings. On the other hand, apoptosis assay for this compound was also carried out by flow cytometry supporting the other results.

کلیدواژه ها:

Unsymmetrical thioureas ، MTT-assay ، Flow Cytometry ، TUNEL assay ، ۱-(۱ ، ۳-benzothiazol-۲-yl)thiourea ، ۳-(benzo[d]thiazol-۲- yl)-۲-thioxo-۲ ، ۳-dihydroquinazolin-۴(۱H)-one