The Effect of Curcumin on GLUT۴ Gene Expression as a Diabetic Resistance Marker in C۲C۱۲ Myoblast Cells

Objective: Adipocyte and skeletal muscle are important tissues which contribute the development and progression of metabolic disorder. Insulin has a major regulatory function on glucose metabolism in these tissues by redistributing glucose transporter (GLUT۴) from intracellular vesicles to the cell surface. Today, due to the side effects of chemical medications attendance to herbal medicines is growing. In this study, the effect of Curcumin extract as main polyphenols in Turmeric on gene expression of GLUT۴ was evaluated. Materials and Methods: Curcumin was extracted using alcohol and chloroform from turmeric powder.TLC chromatography was used to confirm purity of Curcumin extracted. Mouse C۲C۱۲ myoblasts were grown in Dulbecco’s Modified Eagle Medium (DMEM ۱.۵ g/l glucose) supplemented with ۱۰% fetal bovine serum, ۵۰ U/ml penicillin, and ۵۰ μg/ml streptomycin. After differentiating of C۲C۱۲ cells during ۴ days to myotubes, cells were treated separately in the presence of insulin (۱۰۰ nM), Curcumin (۲۵ µM) and co-treatment for ۲۴h.RNA extraction from C۲C۱۲ cells was performed and GLUT۴ expression levels were examined by semi-quantitative RT-PCR. Results: The results showed a significant increase in the GLUT۴expression in Curcumin treatment group, in compare with negative control but less than insulin as a positive control. The synergistic effect of Curcumin and insulin was lower in comparison with insulin as a positive control. Conclusion: Curcumin could increase the gene expression but synergistic effects of Curcumin and insulin is more powerful than Insulin which can be due to the competitive action of insulin and Curcumin in activation of gene expression pathway.

کلیدواژه ها:

Curcumin ، Diabetes ، Expression ، GLUT۴