Influence of Added Vitamin C and Vitamin E on Frozen-Thawed Bovine Sperm Cryopreserved in Citrate and Tris-Based Extenders
محل انتشار: گفتمان پژوهش دامپزشکی، دوره: 2، شماره: 1
سال انتشار: 1390
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 35
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شناسه ملی سند علمی:
JR_VRFAN-2-1_005
تاریخ نمایه سازی: 25 آبان 1402
چکیده مقاله:
The objective of this study was to evaluate quality of frozen-thawed bull semen processed with extenders containing vitamin C and E as antioxidants. Pooled semen's were collected from ۵ bulls and diluted to a concentration of ۳۰ × ۱۰۶ sperm/mL with citrate –egg yolk (CEY) or Tris – egg yolk (TEY) extenders. The diluted semen was divided to ۵ aliquots including control and ۴ experimental groups. Each aliquot was further diluted with an equal volume of CEY or a Tris (hydroxymethyl) aminomethane (TRIS) - based extender without (control) or containing vitamin C ۱mM or ۲mM and vitamin E ۰.۱mM or ۰.۲mM, and routine semen evaluations like sperm motility, viability and measurement of lipid peroxidation (LPO) were conducted. Significant reductions of LPO were achieved by addition of ۱mM vitamin C and ۰.۱ mM vitamin E to CEY extender (P < ۰.۰۵). The highest sperm viability was achieved by addition of ۰.۱mM vitamin E to CEY extender. Supplementing CEY extender with ۲mM vitamin C and ۰.۱mM vitamin E improved the sperm motility compared with the control group. On the basis of the present results it is concluded that vitamin C and E are very efficient antioxidants in CEY extender.
کلیدواژه ها:
نویسندگان
Reza Asadpour
Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Iran
Razi Jafari
Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Iran
Hossein Tayefi - Nasrabad
Department of Basic Sciences, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Iran