Effects of diluting medium and holding time on sperm motility analysis by CASA in ram
محل انتشار: گفتمان پژوهش دامپزشکی، دوره: 5، شماره: 2
سال انتشار: 1393
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 35
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شناسه ملی سند علمی:
JR_VRFAN-5-2_004
تاریخ نمایه سازی: 25 آبان 1402
چکیده مقاله:
The aim of this study was to evaluate the effects of dilution rate and holding time on various motility parameters using computer-assisted sperm analysis (CASA). The semen samples were collected from three Ghezel rams. Samples were diluted in seminal plasma (SP), phosphate-buffered saline (PBS) containing ۱% bovine serum albumin (BSA) and Bioexcell. The motility parameters that computed and recorded by CASA include curvilinear velocity (VCL), straight line velocity (VSL), average path velocity (VAP), straightness (STR), linearity (LIN), amplitude of lateral head displacement (ALH), and beat cross frequency (BCF). In all diluters, there was a decrease in the average of all three parameters of sperms movement velocity as the time passed, but density of this decrease was more intensive in SP. The average of ALH between diluters indicated a significant difference, as it was more in Bioexcell in comparison with the similar amount in SP and PBS. The average of LIN in the diluted sperms in Bioexcell was less than two other diluters in all three times. The motility parameters of the diluted sperms in Bioexcell and PBS indicated an important and considerable difference with the diluted sperms in SP. According to the gained results, the Bioexcell has greater ability in preserving motility of sperm in comparison with the other diluters but as SP is considered as physiological environment for sperm. It seems that the evaluation of the motility parameters in Bioexcell and PBS cannot be an accurate and comparable evaluation with SP.
کلیدواژه ها:
نویسندگان
Somayeh Mostafapor
Department of Animal Science, Faculty of Agriculture, Urmia University, Urmia, Iran
Farhad Farrokhi Ardebili
Department of Animal Science, Faculty of Agriculture, Urmia University, Urmia, Iran
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