Characterization of a novel bullet-shaped lytic bacteriophage against extensively drug-resistant Pseudomonas aeruginosa isolated from human and domestic sources

Global spread and emergence of the extensively drug-resistant (XDR) strains of P. aeruginosa have become a concern, thus, searching for new alternative treatment approaches are required. This study was aimed to isolate and characterize a novel lytic phage against P. aeruginosa. Seventy XDR isolates of P. aeruginosa were collected from May to September ۲۰۱۸. Wastewater samples were used for isolation of lytic phage against XDR P. aeruginosa isolates. Host range, thermal and pH stability, adsorption rate, latent period, burst size and morphology of phage were determined following the standard protocols. Morphological characteristics of the phage revealed that it belonged to Podoviridae family and it was named vB-PaeP-۰۰۷. Although the phage had a narrow host range, ۴۷ out of ۷۰ XDR isolates were susceptible to it. The adsorption rate, latent period and burst size of vB-PaeP-۰۰۷ were approximately ۸۹.۸۰% in ۸ min, ۱۰ min and ۹۳ phages per cell, respectively. Its lysis activity remained at a wide range of pH (۴ up to ۱۲) and temperature (– ۲۰.۰۰ up to ۷۰.۰۰ ˚C). Regarding the physiological features and host range of the vB-PaeP-۰۰۷ phage, it could be a promising candidate for phage therapy and bio-controlling of infections from XDR isolates of P. aeruginosa in human and livestock storage centers.

کلیدواژه ها:

Bacteriophage ، Extensively Drug Resistant ، Phage therapy ، Pseudomonas aeruginosa