Allelic Polymorphism of Makoei Sheep Calpastatin Gene Identified by Polymerase Chain Reaction and Single Strand Conformation Polymorphism

Calpastatin (CAST) is a specific inhibiter of calpains, playing a role in meat tenderization and myogenesis. In the present study, the polymorphism of the CAST gene of Makoei sheep was investigated by polymerase chain reaction and single strand conformation polymorphism technique (PCR–SSCP). Genomic DNA was extracted from whole blood samples collected from ۱۰۰ sheep. A ۶۲۲ bp CAST exon ۱ segment was amplified by standard PCR, using the locus specific primers. PCR products were subjected to a non-denaturing gel electrophoresis. Four SSCP patterns, representing four different genotypes, were identified. The frequencies of the observed genotypes were ۰.۳۱, ۰.۰۴, ۰.۶۳ and ۰.۰۲ for AA, BB AB and AC, respectively. Allele frequencies were ۰.۶۳۱۳, ۰.۳۵۸۶ and ۰.۰۱ for A, B and C, respectively. The Observed heterozygosity (Hobs) value for CAST gene was ۰.۴۷۲۸. The chi-square test showed significant (P< ۰.۰۱) deviation from Hardy-Weinberg equilibrium for this locus in Makoei sheep population.

کلیدواژه ها:

CAST gene ، Makoei sheep ، PCR ، SSCP