Effects of Silicon and AgNO۳ Elicitors on Biochemical Traits and Antioxidant Enzymes Activity of Henbane (Hyoscyamus reticulatus L.) Hairy Roots

Lattice henbane (Hyoscyamus reticulatus L.) is an herbaceous, biennial plant belonging to Solanaceae family. H. reticulatus hairy roots were established from two-week-old leaves infected by A۷ strain of Agrobacterium rhizogenes on solid Murashige and Skoog (MS) medium. In this study, abiotic elicitors including; Sodium silicate (Na۲SiO۳) with different concentrations (۰, ۱, ۵ and ۷ mM) and silver nitrate (AgNO۳) concentrations (۰, ۰.۵, ۱ and ۲ mM) were added to hairy roots culture media. The results showed that, Na۲SiO۳ and AgNO۳ significantly affected hairy roots fresh weight after ۲۴h. Also, the highest hairy root fresh weight was observed in the control, and with broadening elicitor concentrations, fresh weight was decreased in both treated hairy roots with AgNO۳ and Na۲SiO۳ but the effect of exposure duration was not significant. Biochemical analysis showed that total antioxidant activity (TAA), total phenol (TP), catalase (CAT), ascorbate peroxidase (APX) and Guaiacolperoxidase (GPX) activities were enhanced in elicitated hairy roots compared to non elicitated hairy roots. The highest CAT, APX and GPX activities were observed in hairy roots treated with ۷mM Na۲SiO۳ and ۲mM AgNO۳. Our results suggest that, Na۲SiO۳ and AgNO۳ can stimulate the antioxidant defense systems and protect the plants from subsequent stresses.

کلیدواژه ها:

Agrobacterium rhizogenes ، Hyoscyamus ، Abiotic stresses ، Total antioxidant