First Molecular Detection and Phylogenetic Analysis of Ehrlichia canis in Dogs from Baghdad, Iraq

Ehrlichia canis is a pathogen considered a disease in both dogs and breeders, a tropical and non-tropical disease caused by an intracellular pathogen. For the first time, the study aimed at hematological and molecular detection and phylogenetic analysis of Erlchcia canis in dogs in Baghdad, Iraq. Two hundred dogs were clinically examined from April to September ۲۰۱۹ at the Veterinary Hospital in Baghdad. Blood samples with EDTA tubes were used for microscopic examination, complete blood count (CBC) and polymerase chain reaction (PCR). The study's results revealed that the infection rate was ۳.۵% when analyzed using microscopic or molecular methods. Ataxia, posterior recumbency, and occasionally vision problems were identified as the clinical characteristics that were distinguished in this study. The hematological values showed no significant differences between infected and uninfected dogs (P<۰.۰۵). However, the study did show that infected dogs had neutrophilia and monocytosis. Four samples were sent to the sequencers, and NCBI accession numbers were assigned to two isolates of the Ehrlichia canis ۱۶s rRNA gene (MN۲۲۷۴۸۳.۱ and MN۲۲۷۴۸۴.۱). This study showed that ۹۹% of the isolates matched those found in other countries. The study concluded that microscopic examination is not the best method for diagnosing Ehrlichia in dogs because it requires the ability to differentiate microscopically between intracellular inclusion bodies and the included morula of Ehrlichia and may produce incorrect results. Instead, molecular tests are used to confirm an Ehrlichia diagnosis.

کلیدواژه ها:

Hematology ، Ehrlichia canis ، Polymerase Chain Reaction