Impact of Tween ۸۰ on Fatty Acid Composition in Two Bacterial Species

Tween ۸۰ is a well-known non-ionic emulsifier which is used in pharmaceutical and food industries. Due to its widespread applications it is needed to understand how it affects bacteria. The current study aimed to investigate the effect of Tween ۸۰ on the fatty acid content of bacteria.  Three bacterial isolates were used in this study: two isolates of Serratia marcescens and one isolate of Acinetobacter baumannii. The analysis of fatty acid components of bacterial lipids was performed, followed by the assessment of the effect of Tween ۸۰ on fatty acid content by adding it to the culture medium in three concentrations: ۰.۱%, ۰.۵% , and ۱.۰ % .The results indicated that the Tween ۸۰ has the ability to change the fatty acid content present in these bacteria by the appearance and disappearance of three fatty acids, including Elaidic acid ۱۸.۱ trance ۹, Oleic acid ۱۸.۱ cis ۹, and erucic acid ۲۲.۱, which belong to Mono-Unsaturated Fatty Acids (MUFA), in the presence of different concentrations of Tween ۸۰.  The results also demonstrated that the p-value was significant in two situations, the first belonged to S. marcescens at ۰.۵ % concentration for all groups of FAs (Saturated fatty acids, Monounsaturated fatty acids, and polyunsaturated fatty acids), and the second was for S. marcescens just in MUFA in all concentrations of Tween ۸۰.

کلیدواژه ها:

fatty acids ، Serratia marcescens ، Acinetobacter baumanniim ، Tween ۸۰