Evaluation of the Role of the KRAS Gene Polymorphism LCS۶ (rs۶۱۷۶۴۳۷۰) in Iraqi Women with Ovarian Cancer

In carcinogenesis, KRAS is an essential oncogene that plays a key function. The polymorphism of rs۶۱۷۶۴۳۷۰ is a candidate for cancer susceptibility in KRAS۳' untranslated region. The current study aimed to determine the role and impact of the KRAS gene polymorphism (rs۶۱۷۶۴۳۷۰ T>G) on the risk of ovarian cancer development in the Iraqi population. In total, ۸۴ ovarian cancer patients and ۲۸ ovarian benign tumors were involved in a case-control study. DNA extraction from the formalin-fixed/paraffin-embedded tissues, followed by the sequencing of PCR products was carried out in genetic analysis for the detection of the KRAS polymorphism (rs۶۱۷۶۴۳۷۰ T>G). The results showed that the frequencies of the KRAS gene polymorphism (rs۶۱۷۶۴۳۷۰) in ovarian cancer patients were ۷۸ (۹۲.۸۵%) and ۶ (۷.۱۵%) with genotypes homozygote TT and heterozygote TG, respectively. These corresponding values in patients with benign ovarian tumors were ۲۵ (۸۹.۳%) and ۳ (۱۰.۷%) with homozygote TT and heterozygote TG, respectively. None of the patients either with malignant or benign tumors have been detected with homozygote genotype GG. Genotype frequency of the TT and TG showed that the heterozygote TT genotype vs. TG and T allele vs. G allele were more frequent in malignant and benign tumors (P≤۰.۰۱). Statistically, there was no association between the KRAS polymorphism and the clinical characteristics of ovarian cancer patients, such as age, family history, menopause, histological type, tumor size, or tumor stage. In conclusion, a significant association was found between rs۶۱۷۶۴۳۷۰ and the risk of ovarian cancer in the Iraqi population, particularly those with genotypes homozygote TT. On the other hand, genotypes had no relationship with any of the clinical characteristics of ovarian cancer patients. Additional well-designed studies with larger sample sizes are recommended to validate the precise role of KRAS LCS۶ variations in ovarian cancer risk.

کلیدواژه ها:

KRAS ، LCS۶ Polymorphism ، Ovarian cancer ، rs۶۱۷۶۴۳۷۰ T>G