Development of a Nano-ELISA system for the rapid and sensitive detection of H۹N۲ avian influenza

Influenza is one of the most important viral diseases that is common among the birds and the mammalsand is caused by specific viruses that belong to the Orthomyxoviridae family. Migratory aquatic birds arethe reservoir of the disease and there is a likelihood of the disease in any region. There are differentmethods for detecting the avian influenza, but by the point of detection rates, the ELISA may be one ofthe most important current methods. In this work we synthesized Gold nanoparticles and conjugated itwith rabbit-anti-chicken IgG-HRP. An ELISA test was done to compare the bioactivity of Au-antichickenHRP with anti-chicken HRP in order to detect the antibody against the H۹N۲ subtype of avianinfluenza virus. Using ۱۳۳ field chicken sera, the sensitivity of nano-ELISA as compared to traditionalELISA was calculated to be ۱۰۰%, whereas the specificity was ۹۲%. This method was significantly moresensitive than the traditional ELISA and didn’t require extra costs. It can therefore be concluded that theAuNP-HRP conjugate can be applicable in immune analysis procedure where a more confident result isrequired.

کلیدواژه ها:

Avian Influenza ، ELISA ، Gold nanoparticle