Oleuropein Attenuates Deltamethrin-induced Apoptosis in Rat Cerebellar Purkinje Neurons

Background: Deltamethrin (DM) is a synthetic pyrethroid insecticide that can elicit neurotoxicity, and lead to apoptosis. There is accumulating evidence that oleuropein (OE) has anti-apoptotic effect. This study aimed at determining the DM toxicity and anti-apoptotic effect of OE pretreatment in cerebellar Purkinje neurons. Materials and Methods: Rats were randomly divided into four groups as follow: DM treated group (۱۲.۵ mg/kg single dose), OE treated group (۲۰ mg/kg per day), DM plus OE treated group, and vehicle group. Sections of cerebellum were taken ۲۴ hours after deltamethrin injection and studied for histopathological and immunohistochemistry assessments. Results: Further characteristics of degeneration in Purkinje neurons were observed in DM group compared with DM plus OE group. Compared with DM group (۹.۵۶±۱.۶۹), the positive staining for Bax in Purkinje neurones decreased in DM plus OE group (۲.۹۹±۰.۵۰) but upper than OE (۰.۷۲±۰.۱۵) and vehicle (۰.۵۷±۰.۰۳) groups. Compared with DM group (۰.۵۰±۰.۰۵), the positive staining for Bcl-۲ in Purkinje neurons increased in DM plus OE group (۳.۲۹±۰.۱۸) but lower than OE (۴.۳۸±۰.۸۰) and vehicle (۵.۸۷±۱.۹۳) groups. Conclusions: Our results suggest that DM induces apoptosis in Purkinje cells which is subsided by oleuropein.

کلیدواژه ها:

Deltamethrin ، Oleuropein ، Purkinje ، Apoptosis