Synergistic Effects of NDRG۲ Overexpression and Radiotherapy on Cell Death of Human Prostate LNCaP Cells

Background: Radiation therapy is among the most conventional cancer therapeutic modalities with effective local tumor control. However, due to the development of radio-resistance, tumor recurrence and metastasis often occur following radiation therapy. In recent years, combination of radiotherapy and gene therapy has been suggested to overcome this problem. The aim of the current study was to explore the potential synergistic effects of N-Myc Downstream-Regulated Gene ۲ (NDRG۲) overexpression, a newly identified candidate tumor suppressor gene, with radiotherapy against proliferation of prostate LNCaP cell line.Materials and Methods: In this study, LNCaP cells were exposed to X-ray radiation in the presence or absence of NDRG۲ overexpression using plasmid PSES- pAdenoVator-PSA-NDRG۲-IRES-GFP. The effects of NDRG۲ overexpression, X-ray radiation or combination of both on the cell proliferation and apoptosis of LNCaP cells were then analyzed using MTT assay and flow cytometery, respectively.Results: Results of MTT assay showed that NDRG۲ overexpression and X-ray radiation had a synergistic effect against proliferation of LNCaP cells. Moreover, NDRG۲ overexpression increased apoptotic effect of X-ray radiation in LNCaP cells synergistically.Conclusion: Our findings suggested that NDRG۲ overexpression in combination with radiotherapy may be an effective therapeutic option against prostate cancer.

کلیدواژه ها:

Gene Therapy ، Radiation Therapy ، Prostate Cancer ، N-Myc Downstream-Regulated Gene ۲