Hwp۱ gene Expression of Candida albicans and Study its role in adherence
سال انتشار: 1392
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 37
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شناسه ملی سند علمی:
JR_IJMCM-3-2_009
تاریخ نمایه سازی: 16 اسفند 1402
چکیده مقاله:
Candida albicans is an opportunistic fungal pathogen found as mycoflora in the human body surfaces. Sevral genes play a crucial rule in its virulence including Hwp۱ (hyphal wall protein ۱), BCR۱ and ALS gene family. Hwp۱ gene is a responsible for coding a cell surface protein, which mediates biofilm formation in candida albicans. Here we investigated the presence of the HWP۱gene was characterised among Candida albicans isolates in women with recurrent vaginal canididasis and further we studies its role in cell adherence. We used ۵۰ Candida albicans clinical isolates resistant to Fluconazole. RNA (of samples were extracted using glass bead and lysis buffer and cDNA was synthesized using reverse transcriptase enzyme. Expression of (HWP۱) gene was analysed using reverse transcriptase-plolymerase chain reaction (RT-PCR). The ability of adherence of the isolates with or without the expression of HWP۱ were characterized using Hela cells. Statistical analysis were performed using t-test and two-way ANOVA SPSS software. Our results showed that the HWP۱ gene were present in ۴۷ samples (۹۴%) out of ۵۰ isolates, ۲۷ samples (۵۷%) had expression of HWP۱. The result of adherence assay revealed that the isolates with the expression of HWP۱ gene and control isolates was statistically different (p<۰.۰۵). In conclusion, the isolates with the expression of HWP۱ gene has the higher ability to adhere the epithelial mucosal cell surface. Our data support a positive correlation between the expression of HWP۱ gene and the ability of adherence to epithelial cells.
کلیدواژه ها:
نویسندگان
Maryam hosseini por
Department of Medical Mycology, Faculty of Medical Science ,Tarbiat Modares University ,Tehran, Iran
Shahla Roudbarmohammadi
Department of Medical Mycology, Faculty of Medical Science ,Tarbiat Modares University ,Tehran, Iran
Maryam roudbary
Department of Medical Parasitology and Mycology, School of Medicine, Iran university of Medical Science, Tehran, Iran
Bita Bakhshi
Department of Bacteriology, Faculty of Medical Science, Tarbiat Modares University, Tehran, Iran
Zahra Farhadi
Department of Midwifery and gynecology, Faculty of Medical Science, Shahid Beheshti University, Tehran, Iran