Electrospun scaffold of Silk fibroin/Chitisan nanofibers

Tissue engineering is a field for the repair of damaged tissues, which can facilitate the substitution at the damaged sites by engineered tissues with suitable characteristics. In this research, silk fibroin / chitosan nanofibers prepared with electrospinning technique as a tissue scaffold. Silk fibroin extracted from silkworm cocoons in the first step. Then chitosan and silk fibroin polymer dissolved with weight ratio 7:3 in a mixed solvent system of TFA/DCM. The electrospun SF/CS blend nanofibers were collected on a target drum. The voltage in range of 12-18 kV were applied by a high voltage power supply. The beadless nanofibers scaffold were assayed by SEM imaging for determining morphology and average diameter of nanofibers. For degradation invitro assay, Phosphate-buffered saline was used during 84 days. SEM images results showed that the nanofibers diameter were in the range of 80-170 nm. During invitrio assay, more than 95% of total scaffold weight remained. FTIR spectroscopy showed changes on nanofibers surface. These polymers contain suitable functional groups that prepare a lot of domains for cells attaching without surface modification. Slow degradation and hydrophilic surface promoting cell adhesion, good biocompatibility and antibacterial activity of chitosan give suitable features to this scaffold.

کلیدواژه ها:

Silk fibroin ، chitosan ، electrospinning ، nanofiber ، scaffold