Gastroprotective effect of Hydro-Alcoholic extract and Sodden of Spinacia Oleracea (HESO) on Hcl/Ethanol-Induced gastric damage in rats

Introduction: Gastritis is the most common damages in gastrointestinal tract. The current common drugs are useful for treatment of gastritis with some side effects. So, new research more concentrated on medicinal plants due to plants are the richest resources of new drugs for traditional and modern systems of medicine and play an important role in human health with less side effects. The aim of this study was to evaluate the Gastroprotective effect of Hydro-Alcoholic extract and Sodden of Spinacia Oleracea(HESO) on Hcl/Ethanol-Induced gastric damage in rats. Materials and methods: Aerial parts of Spinacia Oleracea was collected and extraction carried out by maceration method with ethanol-water 70% (V/V) solveny at room temperature. The rats were divided into five groups of six each: Group I–Negative control received water (1ml/kg,B.W), Group II–Positive control received Hcl/Ethanol (1ml/kg, B.W), Group III–Treatment 1 received Hcl/Ethanol (1ml/kg, B.W)+Spinacea Olerace sodden (1ml/kg, B.W), Group IV-Treatment 2 received Hcl/Ethanol (1ml/kg, B.W)+HESO (250 mg/kg,B.W), Group V-Treatment 3 receivedHcl/Ethanol (1ml/kg, B.W)+HESO (500 mg/kg,B.W), In groups IV and V the HESO administered orally 4 days prior to induction of gastric damage, then prescription continued by the HESO (250, 500mg/kg,B.W) and Hcl/Ethanol (1ml/kg,B.W) simultaneously for 4 days later, except group III which received Spinacea Olerace Sodden (1ml/kg, B.W) and Hcl/Ethanol (1ml/kg, B.W) simultaneously. Animals were sacrificed under light ether anesthesia after 8 days. Blood was collected by heart puncture for, malondialdehyde (MDA) as a product of lipid peroxidation and nitric oxide radical (NO°) and reduced glutathione (GSH) superoxide dismutase (SOD) and catalase (CAT) and reduced glutathione were measured in serum. Results: Levels of antioxidant enzymes (e.g. SOD and CAT) and GSH decreased as compared to normal control group. MDA and NO radical were increased in positive control group. Although SOD, CAT, GSH, MDA and NO radical have been significantly restored toward normal by HESO (P<0.05). The HESO proved to display a dosedependent gastroprotective effect. Conclusion: The HESO displayed significant protective effect on Hcl/Ethanolinducedgastric damage in rats which may be related to the antioxidant activity of the HESO.

کلیدواژه ها:

Gastric damage ، Ethanol ، Hydrochloric acid ، Sodden and Hydro-Alcoholic extract of Spinacia Oleracea