Analysis of Antibiotic Resistance and Antimicrobial Effects of Enterococcus faecium and Lactococcus lactis Isolated from Khorramabad Traditional Cheeses

Enterococcus and lactococcus are Gram-positive cocci that often occur in pairs (diplococci) or short chains, and are difficult to differentiate from streptococci onphysical characteristics alone. Enterococcus faecium because have concern antibiotic resistant, consider difficult as probiotic. The results of an assay show thata probiotic E. faecium strain might be a potential recipient of Vancomycin resistance genes. For analysis of this concept, 13 samples of traditional cheeseswere collected from different areas in Khorramabad city and identified with using phenotypic methods, and then the bacteriocin was extract from indentified bacteria.Agar diffusion method was used to assay the antimicrobial activities of bacteriocin produced by isolated bacteria against Pseudomonas aeruginosa, Proteus vulgaris, Staphylococcus aureus, E. coli, Bacillus cereus and Bacillus subtilis. On the otherhand, antibiotic resistance of these bacteria was test using antibiogram method. The results showed that some of bacteria such as P. aeruginosa, S. aureus, and E. coliare intermediately resistance while P. vulgaris was completely sensitive and B. cereus and B. subtilis were resistant. Also, the Enterococcus faecium was resistantto kanamycin and trimethoprim antibiotics and intermediatly to clindamycin and tetracycline, and sensitive to amoxicillin and erythromycin. Lactococcus lactis was sensitive to trimethoprin, amoxicillin, tetracycline, and erythromycin while wasresistant to kanamycin and clindamycin. In this study both bacteria enterococcus faecium and lactococcus lactis had an inhibitory effect on pathogenic bacteria and, these bacteria also have an appropriate antibiotic resistance to most antibiotics.

کلیدواژه ها:

Enteroccos faecium ، Lactococcus lactis ، Antibacterial ، Antibiotic