The effects of Piriformospora indica symbiosis with tomato on the expression of some membrane transporter genes and salt tolerance

In this study, the positive role of the Piriformospora indica, an endophytic fungus, in increasing the tolerance of tomato plants to salt stress through the expression of several genes such as NHX3, NHX4, CNGC15, SOS1, PIP1/2, PIP2/4, TIP1/2 and TIP2/2 was investigated. For this purpose, the expression of these genes was evaluated in leaves and roots of the inoculated and un-inoculated tomato plants using real-time PCR technique. The experiments were conducted at 150 mM of salt during 0, 24, 48, 120 and 240 hours of treatment courses. The results showed that in vitro inoculation of tomato plants by the endophytic fungi resulted in the improvement and expression of some genes, such as plasma membrane carriers, NHX3 and NHX4, SOS1 and CNGC15, and reduced the expression of aquaporin genes in the tonoplast.As a general conclusion, it can be said that the endophytic fungus alters the expression of some effective genes of cellular membrane transporters in tomato plants. It can tend to maintain ion homeostasis, diminish sodium to potassium ratio, and an increase in water absorption promoting tolerance to salinity

کلیدواژه ها:

Salt stress ، Tomato ، Priformospora indica ، Transporters