HN-00350051_The Effects of ω-3 Fatty Acids and Nano-Curcumin on the COX-2/ inOS Network Gene Expression, Serum Levels and Clinical Symptoms

Introduction: One of the common Neuro-inflammatory disorders is Migraine. Human and experimental models of migraine studies have indicated the role played by COX-2/ inOS in migraine’s Neuro-inflammatory pathogenesis. COX-2 andinOS are connected and contribute to inflammation and neurogenic painin the central nervous system. Omega- 3 fatty acids curcuminand active polyphenol of turmeric have Neuro-protective and anti-inflammatory effects via different mechanisms, including the suppression of COX-2 andinOS gene expression, as well as their serum levels. The goal is to evaluate the nutrigenomic effects of ω-3 fatty acids, nano-curcumin, and a combination of the two, on neuro-inflammation and clinical symptoms in migraine patients.Methods: This study indicates the results of a clinical trial over a 2-month period, considering 74 episodic migraine patients who took ω-3 fatty acids, nano-curcumin, a combination of them, or a placebo. At the beginning and end of the trial, COX-2/inOS serum levels and the expression of COX-2/inOS (in peripheral mononuclear blood cells isolated from patients) were measured, using real-time ELISA and PCR respectively. The frequency, severity and duration of pain attacks were also recorded.Results: The findings showed that ω-3 fatty acids and nano-curcumin can affect synergistically in the down-regulation of COX-2/inOS mRNA, as well as reduce their serum levels. Moreover, the combination of ω-3 and nano-curcumin significantly reduced the frequency, severity and duration of headaches (P<0.05).Conclusion: The current findings demonstrate that therapy with combination of ω-3 fatty acids and nano-curcumin can be considered as a new approach in migraine prevention.

کلیدواژه ها:

curcumin ، COX-2 ، inOS ، headache ، Migraine ، ω-3 fatty acids