Simultaneous effect of static magnetic field and doxorubicin on exosomal genes expression and oxidative stress in colorectal cancer cells

Background and Aim: Doxorubicin (Dox) works as a chemotherapeutic agent used in treating solid tumors. Dox has two distinct mechanisms. Firstly, it works as an intercalator, enters DNA molecules and inhibits topoisomerase II activity in DNA repair. Secondly, it reactivates oxygen species (ROS) production which causes DNA damage, protein oxidation, lipid peroxidation, and cell apoptosis. SMF exposure increases Dox uptake by cells and also the activity, concentration, and half-life of ROS which enhances Dox chemotherapeutic activity. Therefore to increase Dox effects and decrease its side effects, we used both Dox and static magnetic field (SMF) simultaneously on colorectal cancer cell line HT۲۹.Methods: Inhibition of cell growth in response to Dox - SMF was assessed by MTT assay. Total ROS was measured by ۲′, ۷′-dichlorodihydrofluorescein diacetate (DCFH-DA). Dox uptake was measured by Dox fluorescence intensity at ۵۹۰ nm. The qRT-PCR was performed using the fluorescent dye SYBR Green. The relative expression was calculated using the ۲-ΔCT method.Results: The results indicated that the dosage of ۵-۲۰ mT of SMF can cause cell proliferation by producing ROS in very low concentration. But Dox increases the ROS level. Dox -SMF combination also increases ROS production and also increase Dox uptake by the cells. Dox -SMF combination also decreases Dox LD۵۰ and cell viability. Dox-SMF combination also increases Dox uptake by the cells. qPCR results illustrated that Dox decreases CD۶۳, RAB۲۷a and ALIX expression but SMF increases them. SMF-DOX combination also decreases CD۶۳, RAB۲۷a and ALIX expression.Conclusion: SMF can decrease the required dose of chemotherapy drugs such as Dox and therefore decreases their side effects.

کلیدواژه ها:

Colon cancer ، Doxorubicin ، Static magnetic field ، ROS