Association analysis for traits associated with powdery mildewtolerance in barley [Hordeum vulgare L.] using AFLP markers

Association analysis is a useful method for evaluation of significant association between molecularmarker and phenotype of trait. This study was performed to evaluate association between traits relatedwith powdery mildew resistance and molecular markers. This investigation was performed using 77barley genotypes and AFLP markers. In phenotypic evaluation, reaction of seedlings to powdery mildewwas evaluated and the infection type and intensity were assessed based on 0-9 scale as the most importanttraits associated with resistance. Also in this study, the genetic diversity of genotypes was evaluated usingseven combination primers EcoRI/MseI. The average percentages of polymorphism and polymorphicinformation content were 92.37% and 0.43, respectively. General evaluation of the statistics of geneticdiversity showed that among seven primer combinations, three combinations of E90-M160, E100-M160,and E100-M150 were higher value than others and had a more obvious effect in the detection andseparation of barley genotypes. Association analysis was performed using four statistical models of GLMand MLM applying TASSEL software. In the complete MLM model, 33 markers showed significantassociation in the 5 percent probability level with traits and the highest coefficient of determination wasrelated to marker E80-M150-3 that explained 14% of variations of infection intensity. E80-M510-3 andE80-M160-22 markers were showed significant association (pr<0.05) with both characteristic the severityand type of infection that can represent the effective role of this genomic region in resistance to powderymildew. If the results are confirmed, it can be a suitable candidate for conversion to SCAR specificmarker.

کلیدواژه ها:

AFLP marker ، Association analysis ، Barley ، Fungal disease ، Powdery mildew