Evaluation of Agrobacterium-mediated Transformation of Chlamydomonas reinhardtii Using a Synthetic Amorpha-4, 11-diene Synthase Gene
محل انتشار: مجله سلول و تحقیقات مولکولی، دوره: 7، شماره: 1
سال انتشار: 1394
نوع سند: مقاله ژورنالی
زبان: فارسی
مشاهده: 504
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شناسه ملی سند علمی:
JR_JCMR-7-1_001
تاریخ نمایه سازی: 7 بهمن 1395
چکیده مقاله:
Amorpha-4,11-diene synthase (ADS) is a key enzyme in biochemical pathway of the antimalarial agent artemisinin. An Agrobacterium-mediated transformation was carried out to express a synthetic ADS gene in green microalgaChlamydomonas reinhardtii strain 125C with bacterial strains GV3101 and LBA4404. The foreign gene wasoptimized based on codon usage bias of the microalga. Integration of the ADS in nuclear genome of C. reinhardtiiwas confirmed by polymerase chain reaction assay. The transgenic colonies cultured on selective medium turned yellow after three days and gradually died. Transformation procedure, growth habit of the transgenic microalgae together with probable causes of transformants loss is discussed. The present study is the first investigation for production of ADS enzyme in a microalgal system
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نویسندگان
Afsaneh Mohkami
Biotechnology and Plant Breeding Department, College of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran
Hassan Marashi
Biotechnology and Plant Breeding Department, College of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran
Farajolah Shahriary Ahmadi
Biotechnology and Plant Breeding Department, College of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran
Masoud Tohidfar
Tissue Culture and Gene Transformation Department, Agriculture Biotechnology Research Institute of Iran (ABRII), Karaj,Iran